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The cells were removed from the incubator, the mixture of PI and Hoechst was discarded, and the cells were rinsed three times with PBS

Preservative allows for multiple safe withdrawals

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Sator-Katzenschlager SM, Szeles JC, Scharbert G, Michalek-Sauberer A, Kober A, Heinze G, et al

A highly-trained, certified infusion specialist can administer IV fluids