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The membrane was blocked with 3% (w/v) milk in TBS-T [20 mM TrisHCl (pH 7.5), 150 mM NaCl, 0.1% (v/v) Tween-20] for 30 min at RT and incubated with primary antibodies (diluted in 3% milk/TBS-T) overnight at 4 C

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However, the process encountered several challenges, including the gap between design and implementation phases, the exclusion of design actors during implementation and significant changes to the proposed designs

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