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Gene expression analysis TMets (GFP + ) were dissected from Apc-Ras-Sns flies between 3 and 4 weeks after induction and incubated for 15 min at 65 C in Lysis buffer (20 mM DTT, 10 mM Tris.HCl ph 7.4, 0.5% sodium dodecyl sulfate, 0.5 g/l proteinase K)

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