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Chan K, Sathyamurthi PS, Queisser MA, Mullin M, Shrives H, Coe DM, et al

Endothelial Functions

A phase ib study of the dual PI3K/mTOR inhibitor dactolisib (BEZ235) combined with everolimus in patients with advanced solid malignancies

Lipid droplets can be visualized using Nile red dye, which stains neutral lipids (Fig 4H)

Blots were then probed with the relevant primary antibodies in 5% BSA, 0.1% Tween-20 and 1 PBS or in 5% non-fat skimmed milk powder in 0.1% Tween-20 and 1 PBS at 4 C overnight with gentle motion in a hand-sealed transparent plastic bag