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DIAGNOSTIC CRITERIA FOR VITAMIN B12 DEFICIENCY USED IN STUDIES ASSESSING THE VITAMIN B12 STATUS IN PATIENTS WITH T2D ON METFORMIN THERAPY Despite the diagnostic specificities of vitamin B12 deficiency and the necessity to measure biomarkers without suggestive symptoms, most studies assessing the risk of vitamin B12 deficiency in metformintreated patients rely solely on total vitamin B12 levels to confirm the diagnosis

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The exact minimum effective concentration that recapitulates our observed suppressive ILC2 phenotype in vivo , remains to be determined, and will likely rely on a complex grouping of factors that take into account systems with endogenous melatonin rhythms (such that were absent in our studies both in vitro and in vivo with BALBc mice)