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Cassis, L

After buffer addition, samples were vortexed to ensure complete lysis and then incubated in the dark at room temperature for 20 min to allow for the alkylation of exposed cysteine residues

But it also means the peptide is unusable in its powdered form

Ensuring an adequate supply of amino acids is crucial because they serve not only as building blocks for bone matrix and muscle but also as signaling molecules that regulate bone turnover