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An Afe I restriction site directly upstream of the Strep -tag II, together with the unique Xba I restriction site in front of the whole reading frame, was subsequently used to exchange the entire coding region for sfGFP by the ones for different other POIs (see Supplementary Table S1)

Front Mol Neurosci2022 Mar 2;15:762544

Khatibi VA, Salimi M, Rahdar M, Rezaei M, Nazari M, Dehghan S, et al

The outcomes from a similar study also demonstrated that changes in temperature/CO 2 levels in stored coffee beans and coffee-based media attributed to OTA production in A

Absorption levels at 570 nm and 650 nm wavelengths were measured by a Berthold TriStar2 S Multimode Reader, and values were normalized to non-treated cells

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