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Studies have consistently reported that inhibiting NNMT activity or downregulating NNMT expression leads to significant increases in NAD + levels in mouse adipocytes (Kraus et al., 2014

Briefly, a chilled methanol extraction of the whole cells or microsome fractions was freeze-dried and resuspended in 1% acetonitrile, and then applied to a Hydrosphere C18 column (YMC) on a Prominence nano HPLC system (Shimadzu, Kyoto, Japan) in line with a 4000 QTRAP mass spectrometer (AB Sciex Instruments, Foster City, CA) equipped with Hydrosphere C18 column (YMC)

The results revealed that KI supplementation to activated B cells increased the Annexin V + 7-AAD + late apoptotic cell population, but not the Annexin V + 7-AAD - early apoptotic cell population (Fig

3, Santana Codina, N

Life Sci 80(6):522529

Subsequently, CHAC1 has been extensively used as a ferroptosis marker, both in vitro and in vivo