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A 1 mL aliquot of each urine sample was first incubated with 200 uL ethyl hydroxylamine hydrochloride (75g/L) at 60oC for 30 minutes to convert keto-acids to their ethoxime derivatives

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Thus, 275 mg/m 3 Tolerable concentration = = 0.3 mg/m 3 (300 g/m 3 ) 1000 where: 275 mg/m 3 was the NOEL for irritation in rats in a 4-week study (Appelman et al., 1986) and 1000 is the uncertainty factor (10 for interspecies variation, 10 for intraspecies variation and 10 for a less than long-term study and severity of effect, i.e., carcinogenicity associated with irritation)