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Enzymatic synthesis of HMFS can be performed in either organic solvent or solvent-free system, but due to the limitation of reactants solubility, many related researches were carried out in solvent system (Table 6)

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Diluting to 2:1 (2 mL per 10 mg) drops osmolarity to approximately 280320 mOsm/L, near-isotonic with interstitial fluid, which reduces post-injection stinging by 6070% based on user-reported pain scores

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