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It was first discovered in surviving neurons of Alzheimer's disease patients, sparking research into neuroprotection and age-related cellular pathways

258 Tetramethylpyrazine Tetramethylpyrazine (TMP, Figure 9), also known as ligustrazine, is a compound originally isolated from the Chinese herbal medicine chuanxiong

On the last day, ethanol-fed mice were given a single gavage of ethanol (5 g/kg b.w., 31.25% ethanol), while the pair-fed mice were given an isocaloric gavage of dextrin maltose

20 g samples were denatured in 2Laemmli buffer, heated at 95 C for 8 minutes, separated on 420% Tris-glycine gels (Bio-Rad), and transferred to nitrocellulose membranes