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Besides the need for larger sample sizes and confirmation studies, there is also a need to clarify what the ideal stimulation parameters are and what the patient pre-requisites are for benefitting from stimulation

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This mismatch between ideal diagnostic conditions and real-world practice contributes to false-negative assessments (Goncalves et al., 2010)

Upon the release of zonulin into the lumen as a result of intestinal exposure to gliadin, zonulin can interact with two receptors, epidermal growth factor receptor (EGFR) and protease-activated receptor 2 (PAR2), which results in increased intestinal permeability (27, 32)