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revealed that polarization-dependent fluorescence correlation spectroscopy (Pol-FCS) can be used for evaluation of macromolecular crowding and simultaneously measure the relaxation times of rotational and translational diffusion of fluorescent molecules at the same position, even in living cells (Yamamoto et al., 2021)

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In contrast, low stiffness increases the activity of the Ras-related GTPase RAP2 which inactivates RhoA and induces the phosphorylation of the large tumor suppressor kinase 1/2 (LATS1/2)