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For hPrdx6 protein purification, the crude protein extract was passed through the Ni-NTA agarose column pre-equilibrated with binding buffer (20 mM Tris (pH 7.0), 50 mM NaCl, 10 mM Imidazole), followed by washing and elution using wash buffer (20 mM Tris (pH 7.0), 50 mM NaCl, 60 mM Imidazole) and elution buffer (20 mM Tris (pH 7.0), 50 mM NaCl, 500 mM Imidazole), respectively

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