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In contrast, the lower chambers were supplemented with RPMI 1640 or McCoys 5 A medium supplemented with 15% FBS to detect tumor cell migration and invasion ability

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Similarly, RNAi of Chop mRNA greatly attenuated MGST2 and NOX4 expression at the protein level, whereas NOX2 expression was not regulated by CHOP (Supplementary Fig

Lower concentrations (e.g., 1mg/mL using 5mL water) improve measurement accuracy but require larger injection volumes and may reduce stability due to increased surface area exposure to oxygen