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This paradigm offers high standardization, reproducibility, and low mortality, and reliably produces anxiety-/depressive-like phenotypes along with HPA axis and inflammatory changes

Samples are then rinsed inPBST2 times for 10 minutes each then incubated in alexa-488 conjugated secondary antibodies for 2 hours at room temperature or overnight at 4 C

The Lancet Neurology, 8(4), 382397

BPC-157 is sold as a research compound and is not FDA-approved for human use

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Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts