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Compared with those in MCF-10A, the mRNA and protein expression of EGR1 in breast cancer cell lines were decreased (Figure 2A-C)

Visceral Fat, Metabolic Syndrome, and Why Selective Reduction Matters Visceral fat is metabolically distinct from subcutaneous fat: it secretes inflammatory cytokines, drives insulin resistance, and is independently associated with cardiovascular disease risk in a way that subcutaneous fat is not

E_eung, D.W., et al, Technique, 1:11-15, 1989

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The cell pellet was resuspended in 1 ml of binding buffer (25 mM Tris-Cl, pH 8.0, 200 mM NaCl) containing 1% Triton X-100 and incubated at 37C with constant shaking at 800 r/min using a thermomixer (Eppendorf) for 2 h