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Serum B12 levels between 148258 pmol/L represent a 'grey area' where deficiency may still be present if symptoms are evident

Post incubation, the liberated IGF-1 was co-immunoaffinity extracted with the internal reference, using protein A/G MSIA pipette tips (from Thermo Fisher Scientific) loaded with antihuman IGF-1 antibody

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Under sub-optimal conditions warm ambient temperatures, frequent puncture without consistent aseptic technique, light exposure the effective preservation window may shorten to less than 28 days even though the calendar window has not elapsed

But Im not holding my breath