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Briefly, samples were mixed with Tris-EDA-HCl buffer and pyrogallol (1:15:1) and incubated at 25 C for 10 min

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For instance, oxidative stress disrupts redox-sensitive organelles, including the endoplasmic reticulum (ER) [9], leading to the accumulation of misfolded proteins, which triggers ER stress and impairs protein folding, modification, and transport [10]

Our results indicate that in a rat model of experimental IR, NAC administration after the induction of a hyperglycemic state provided little protection of the salivary gland mitochondria against free radical injury, apoptosis, and inflammation

Experimental Neurology

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