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Doublets were excluded via FSC-H/FSC-A, lymphocytes gated as CD45 + /SSC-A low, and CD3 + CD4 + T cells identified

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In vitro fertilization and embryonic development analyses COCs after IVM were collected and transferred to HEPES-synthetic oviductal fluid (H-SOF) medium supplemented with 400 IU/ml hyaluronidase

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Methanogens and hydrogen sulfide producing bacteria guide distinct gut microbe profiles and irritable bowel syndrome subtypes