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Clean the injection site with alcohol, allow to dry, pinch the skin lightly if needed, insert the needle at a 45 to 90 degree angle depending on tissue depth, and inject slowly

2 cm) was placed in buffered formalin (pH 7.4) immediately after removal from the animal sacrificed at 24 h after surgery or alternatively, in those rats that received medication at 24 h after surgery at 3 minutes after medication

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Always taper off under medical guidance