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Brain Imaging Behav 14(3):653667

Pires et al., 2022)

Unlike replacement of solutions by microperfusion (from 1 mM to 100 mM glutamate), EPSCs recovered with much faster kinetics upon glutamate uncaging with a time constant of ~15 s, which is 10100 times faster than those estimated in isolated vesicles (Naito and Ueda, Figure 3 Despite this first success toward estimating the rate of glutamate transport in living synapses, there remain several critical concerns regarding the protocol

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