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Recent molecular dynamics studies suggest that during ferroptosis, membrane thinning drives a vicious cycle of increasing access by oxidants, ultimately resulting in increasing membrane curvature and membrane damage through micelle formation [50]

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Specifically, they contain 50 times less catalase compared to hepatocytes, making them more vulnerable to oxidative damage

Subsequently, the cells were permeabilized using 0.1% Triton X-100 prepared in PBS for 10 min