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Fasting is usually unnecessary, although some clinicians prefer avoiding supplements shortly before testing.

Molecular docking analysis The structure of PPAR (PDB ID: 4BCR) was retrieved from the RCSB PDB Protein Data Bank ( Additionally, the structure of indole-3-propionic acid (IPA, chemical component identifier: IOP) was obtained from the Ligand Expo of the Protein Data Bank ( The docking of these two molecules was conducted using HDOCK as previously described 44 , and the results were visualized using PyMOL (v3.2)

doi: 10.1186/s40168-022-01255-6 35

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She explains that the SUSTAIN and PIONEER trials were designed to determine the effectiveness of semaglutide, not the drugs safety profile