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The common disadvantage of these studies is that they only include a small number of cases

2020;7(3):e697

Free Radical Biology and Medicine, 29(34), 222230

And you know that in large numbers

Ramadan fasting is a unique form of intermittent fasting from dawn to sunset (dusk) without eating or drinking during the month of Ramadan based on the lunar calendar 16 and has several major unique features: (1) Fasting is exclusively practiced during the human activity hours from dawn to sunset (dusk) and is for both eating and drinking, which differentiates the dawn to sunset intermittent fasting from the other forms of intermittent fasting where drinking and/or eating light meals are allowed during the fasting window

Ideally, we would like to use tissues from nucleus accumbens core (NAc), a region responsible for locomotor behavior, for this co-immunoprecipitation experiment, but NAc alone might not yield enough proteins to practically perform this assay