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Assessment of apoptosis U937 cells (5 10 5 ) (the two hGH-transfected and the control lines) in 0.5 ml of culture medium were cultured for 48 h in the presence or absence of TNF- (Innotest, Besanon, France) with or without 400 ng ml 1 of cycloheximide (CHX, Sigma, St Louis, MO, USA), as previously reported (Cossarizza et al, 1995)