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After an additional three TBST washes, protein was visualized with SuperSignal West Dura Extended Duration Chemiluminescent Substrate (ThermoFisher) reagents following the manufacturers instructions and imaged using a ChemiDoc-It Imaging System (UVP, Upland, CA) with VisionWorks software

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The requirement for the homeostatic mechanism to be robust means that it must successfully prevent runaway synaptic dynamics across a broad range of input patterns and postsynaptic firing of electrophysiologically heterogeneous inhibitory neurons equipped with diverse plasticity mechanisms