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Cover slips were then blocked in 150 L of blocking solution (5% [w/v] BSA, 5% [v/v] Normal Goat Serum [Jackson ImmunoResearch Laboratories] in 1 PBS) at RT for 90 min

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For GHK-Cu, the analysis should detect a prominent peak corresponding to the expected molecular weight of the GHK-Cu complex (~403.9 Da for the [M+H] adduct)

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The important nuance is that energy is a pathway to behavior change, not a direct metabolic weapon against fat

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