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Following attachment, chromosome spreads were fixed in 2% PFA in PBS for 1520 min, permeabilized with 0.5% Triton X-100 in PBS for 10 min, blocked with 3% BSA in PBS, and incubated with primary antibodies for 2 h at room temperature

Adequate protein intake provides the amino acids needed to build glutathione

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In metabolomics, significant alterations were observed in metabolism related to arginine and proline, arachidonic acid, glutathione metabolism, and ubiquinone and other terpenoid-quinone biosynthesis pathways as CKD progressed with further metabolomic analyses revealing that variations in the distributions of pro-oxidant and toxic metabolites from the four identified metabolic pathways were observed in the feces and serum of the patients as the disease progressed