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doi: 10.1016/j.beem.2004.03.003 55 KrassasGEPoppeKGlinoerD

The peptides were eluted with different concentration of Acetonitrile with 0.1% of formic acid: a linear 180 min gradient of 5 to 28% acetonitrile followed by a 15 min gradient of 28% to 95% and 25 min at 95% acetonitrile with 0.1% formic acid in water at flow rates of 0.15 l min 1