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After two washes in tap water for 5 min, sections were incubated in a solution of 0.1% ammonia water (1 mL ammonium hydroxide in 1 L distilled water) for 1 min, washed again in tap water for 5 min, and counterstained in eosin y solution (SigmaAldrich) for 30 s

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Concentration-dependent cytotoxicity was observed as 5800 M gave a lower cell viability as compared to 580 M