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The increase in plasma MDA in those patients may be attributed to H.pylori -induced chemotactic factors that attract and activate neutrophils and monocytes [8,9] resulting in the generation of ROS in the circulation and gastroduodenal mucosa [10,11]

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The serum was then incubated with PVDF membrane containing 1 mg immobilised antigen overnight at 4 C before affinity-purified with an IgG elution buffer (21004, Thermo Fisher Scientific, MA, USA)