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Bacterial operons generally contained the basic machinery for FeS cluster formation, consisting of a cysteine desulfurase that liberates sulfur for incorporation into nascent clusters (NifS or IscS), a scaffold protein upon which nascent clusters assemble (NifU or IscU), a ferredoxin which likely provides electrons for FeS cluster formation, and a chaperone-co-chaperone pair (HscA and B) that likely aid folding or cluster transfer (reviewed in Johnson et al

[13] Subsequent work on primary human muscle cell cultures found that the MGF-24aa-E peptide increased the proliferative lifespan and delayed senescence of satellite cells isolated from neonatal and young-adult muscle, though notably not from old-adult muscle

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Think of it as routine maintenance for your protocol, the same way you would service a car you drive every day