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When OD 600 reached 1520, cells were collected by centrifugation and resuspended with BMMY medium (replacing glycerol in BMGY with 0.5%(v/v) methanol) to OD 600 of 34 for protein expression

We then measured MDA levels in different intervention groups, and MDA levels increased significantly in the RSL3 and TST groups but were reduced by Fer-1 (Fig

TGR Enzymatic Assays Assays were performed at 25 C in 0.1 M potassium phosphate (pH 7.4), 10 mM EDTA using 100 M NADPH unless otherwise stated