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Samples were stored at 20C for nitrite and leptin assays and at 80C for ghrelin and interleukin-6 (IL-6) analyses

Side effects from injections: Injections are sometimes prescribed for people who have long term conditions which affect the bodys ability to absorb, store, and use B12 - these can last for a persons lifetime

Ceramides, which are elevated in both GWS and ME/CFS, are implicated in the activation of pro-inflammatory cytokines, including interleukin-6 (IL-6) and tumor necrosis factor (TNF-) within immune cells such as macrophages, as well as in serum (Germain et al., 2020

Employing melatonin as an immunomodulatory agent therefore may require higher dosages to be effective, for example in murine models, a dose of 10mg/kg is widely used to investigate the anti-inflammatory and tissue-protective actions of melatonin (70)

Proc Natl Acad Sci U S A (2004) 101(1):296301.10.1073/pnas.2434651100 59 RenCTongYLLiJCDongNHaoJWZhangQHet alEarly antagonism of cerebral high mobility group box-1 protein is benefit for sepsis induced brain injury