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Cells were seeded on collagen-coated plates (250 g/ml rat collagen, Roche) at a density of 150,000 cells/cm 2 and cultivated for 3 h in Williams E medium (PAN Biotech) containing penicillin (100 U/ml, Gibco), streptomycin (0.1 mg/ml, Gibco), gentamycin (10 M, PAN Biotech), dexamethasone (100 nM, Sigma), stable l-glutamine (2 mM, Sigma), insulin supplement (2 ng/ml, Sigma), and 10% Sera Plus (PAN Biotech)

Equal amounts of protein were subjected to SDS gel electrophoresis and transferred to nitrocellulose (NC) paper

Originally known for their role in fitness and anti-aging, these compounds are now gaining prominence for their proven effects in reducing body fat

Acute and chronic hyperkalemia continue to present as major medical dilemmas for healthcare professionals

Tretyakova, Zh

Reverse Aging With Hydrolyzed Collagen Collagen is the primary structural protein in the human body