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The cells were then treated with culture medium containing DOX, MND, ART, MND-ART, or MND-ART-GEL, with final concentrations of DOX and ART of 240 ng/mL and 1.92 g/mL, respectively

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Luminescence was read by a victor3 1420 multilabel counter (Perkin Elmer)

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Initial human tissue analysis revealed that FOXO4 is specifically expressed within Leydig cells of the human testis, as confirmed by its co-expression with Leydig cell markers StAR and CYP11A1 through immunofluorescent staining

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