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The risk of comorbidities, including T2DM, cardiovascular disease, dyslipidemia, and atherosclerosis, is independently correlated with visceral fat mass that is inadequately distinguished by BMI or total body fat

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The filtrate was collected and re-filtered through Whatman paper No

After three washes with PBS (10 min each), the tissues were blocked with 10% normal goat serum for 30 min at room temperature