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To construct a diploid homozygous strain expressing URA3 as the counter-selectable marker in the BY4743 background, first pGSH1 sequence (0585 bp upstream of the GSH1 ORF) and HS fragments for URA3 were amplified from yeast genomic DNA

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Intracellular barriers to non-viral gene transfer

The etiology of PD is multifactorial and involves environmental and genetic factors, aging, and chemical exposure [81]

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But those levels fall by half by age 50 and continue to dip with age