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The recombinant lentivirus was then used to transduce U87MG and U138MG cells, and selection was performed with incubation for 2 weeks with puromycin (1.5 g/ml), resulting in the stable-expressing luciferase glioma cell lines U87-Luc and U138-Luc

3a,b), further confirming that the replicated tests in different times and the culture media would not influence the PCA results (Fig

While the time frame of a single treatment varies from person to person, a session typically lasts 30 45 minutes

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In research, especially with potent compounds like peptides, the dose makes the poisonor the breakthrough