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NADPH, a major cellular reductant, supports the enzymatic activity of FSP1, which resides on the plasma and outer mitochondrial membranes

Using these enzyme preparations with a defined GSH concentration (20 M) in the established two-step in vitro reactions, we found that the extent of conversion of the ultrafiltered flavan-3,3,4-triol ( 4 ) depended on the applied concentration of the purified Pt GSTF8, confirming that this enzyme is required and sufficient for the observed conversion to cyanidin (Extended Data Fig

Plant 8 , 12741284 (2015)

[DOI] [PMC free article] [PubMed] [Google Scholar] 21.Cesarec V, Becejac T, Misic M, et al

We did not receive any comments on our proposed assumptions for the submission of Promoting Interoperability data

This does not make these therapies inherently less valuable but it sets appropriate expectations about the ongoing nature of maintenance