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In cognitive peptides: substitute when possible in vivo tests by cerebral organoids or primary neuronal cultures ( Replacement ), optimise experimental plan to minimise the number of animals ( Reduction ), use appropriate anaesthesia and analgesia during invasive procedures ( Refinement )

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Thereby, it is important to indicate that with consistently less nerve damage in all damaged areas in all BPC 157-cuprizone rats, the most important evidence is the consistent beneficial effect of BPC 157 also in those areas that were most affected, and thereby markedly improved gross presentation and functions in BPC 157 rats

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Furthermore, endotoxin penetration of cell membranes requires accessory proteins such as LPS-binding protein (LBP) and the cell-surface protein cluster-of-differentiation (CD) 14 [52]

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