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However, if the accumulation of acetaldehyde occurs faster than the action of aldehyde dehydrogenase (such as during a heavy drinking session), this can result in a bad hangover

Deffains and Bergman, 2018)

One approach is to position the GST tag at the N-terminus or C-terminus of the target protein, depending on which end is less likely to affect the protein's function

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CPPs represent a diverse and growing catalog of small peptides that have already demonstrated great utility in facilitating the delivery of proteins (Kurrikoff et al., 2021), mRNAs (Yokoo et al., 2021), and small molecules (Tian and Zhou, 2021) to mammalian cells (Shoari et al., 2021)