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Insulin, leptin, and circulating peptide YY have a negative effect

Makhlouf S, Messelmani M, Zaouali J, Mrissa R

This aids in protecting skin cells from damage, supporting structural integrity and helping slow visible signs of premature ageing

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Cell pellets were washed in DPBS with Ca 2+ , Mg 2+ , centrifugated at 51 g for 5 min and resuspended in Williams E medium supplemented with 10% FBS (Merck), 15 mM HEPES, 1 mM sodium pyruvate, 1% penicillin/streptomycin, 1% MEM NEAA (all provided by Gibco), 1 g ml 1 dexamethasone (Jenapharm) and 32 U l 1 human insulin (Eli Lilly)

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