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This in vitro fertilization (IVF)-based technique involves culturing a fertilized egg containing the pathogenic mtDNA mutation until it reaches the 68 cell or blastocyst stage

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Reactions were performed in clear 96-well microplates, initiated by injecting 5 L of ATP into 20 L of a solution containing helicase and nucleic acid such that final concentrations were 25 mM MOPS, 2 mM MgCl 2 , 5 nM NS3h, 0.01 mg/ml BSA, 0.07% Tween20

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