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On day 3, fresh medium containing the cocktail was added to the cell cultures, replacing 1.4 ml of the old medium

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The peptide typically remains stable for 30-45 days when refrigerated

For GHK-Cu pretreatment, mice received intraperitoneal injections of GHK-Cu of 1 or 10 g/g every 24 hours for three days prior to LPS administration

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