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Quantitative real-time PCR analysis Total RNA was isolated from 50 mg liver tissue using TRIzol Reagent (Invitrogen, 15596026) with mechanical homogenization (Precellys Evolution, 3 30 s pulses at 6500 rpm), followed by chloroform phase separation (12,000 g , 15 min, 4 C) and isopropanol precipitation

Snippert, H

A Comprehensive Overview of the Role of Oxidative Stress and Antioxidants in Cardiovascular Diseases

doi: 10.1007/s00213-013-3361-9 58 GaiB

Mechanistically, we found that 4-OI inhibits GPX4-dependent lipid peroxidation by increasing the accumulation and activation of Nrf2

Survivors younger than 10 years at diagnosis had a statistically significantly lower estimated IQ and processing speed scores than older patients