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Intracellular ROS were measured using an ROS detection kit (Invitrogen, CA, USA) following manufacturers protocol, as previously described 31

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Conversely, for Group 3 DEGs ( Tbx1 +/ -unique genes), we identified pathways involved in the fatty acid metabolism (mainly down-regulated genes) and axon guidance process (up-regulated genes) (Fig 5B)

Cell Signaling Technology, 9102), phospho-AKT (Ser473