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A phase 2a pilot demonstrated safety and favourable improvements in liver function compared with matched SOC controls [104]

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The shRNA sequence (TRCN0000322995) targeting ATG10 was designed according to MISSION shRNA Plasmid DNA (MERCK) and synthesized and joined the FastDigest BshTI/FastDigest EcoRI linearized (Thermo Fisher) pLKO.1-GST DAF vector with T4 DNA ligase (EL0011, Thermo Fisher)