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For each independent in vitro experiment, at least three biological replicates were used

This leads to drastically reduced proliferative capacity and cytotoxicity in tumor-infiltrating CD8 + T cells and NK cells due to insufficient metabolic substrates, rendering them poorly responsive to immune checkpoint blockade

This makes it a partly water- and partly oil-soluble material, meaning it functions as an emulsifier helping oil and water to mix

As more data is collected, it is likely that additional vaccine doses may be recommended for those with severe immunosuppression and insufficient responses to previous doses

Together, these strategies aim to build an integrated framework spanning mechanistic studies, targeted delivery systems, and clinical validation, ultimately advancing ferroptosis-based therapies toward precision treatment for ALS

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